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Endocytosis is not required for mislocalization of Pma1 and Can1 in vma3Δ cells. vma3Δ cells expressing Ste3-GFP (A), Pma1-GFP (B), and Can1-GFP (C) were treated with 5 μm Lat-A (+) or dimethyl sulfoxide (−). After 3 h, GFP fluorescence and DIC were imaged and shown in top and bottom panels, respectively.
