FIGURE 7.
Exocytosis and ER-Golgi transport are unaffected in vma3Δ cells. A, secretion of invertase (upper panel) and Bgl2 (lower panel) was detected by Western blot. sec14–3 is a control for a secretory block. I and E are intracellular and extracellular fractions, respectively. B, vacuolar delivery of newly synthesized CPY. Cells were pulse-labeled with Expre35S35S for 5 min and chased for various times. CPY was immunoprecipitated from lysate and analyzed by SDS-PAGE and fluorography. ER-localized p1, Golgi-modified p2, and proteolytically processed mature forms of CPY are indicated.