FIGURE 5.

Soluble corin fragments from the S985A and R801A mutants. A, illustration of WT corin, active site mutant S985A, and activation cleavage site mutant R801A. Soluble corin fragments from transfected HEK 293 (B) and HL-1 (C) cells expressing WT corin and the S985A and R801A mutants were analyzed by Western blotting (left panels). Cell lysate was included as a control for corin expression levels (right panels). Data are from representative experiments that were repeated at least three times.