FIGURE 6.

Corin autocleavage sites. A, illustration of Arg residues in the Fz1 and LDLR5 domains that were substituted by Ala in single mutants. B, soluble corin fragments in the medium from HEK 293 cells expressing WT corin and mutants were analyzed by Western blotting. The S985A and R801A mutants, in which autocleavage was abolished, were used as controls. The ∼160- and ∼100-kDa bands are indicated by an arrow or arrow head, respectively. Data are from representative experiments that were repeated at least three times.