FIGURE 7.

The activity of soluble corin fragments. A, illustration of soluble corin fragments. B, pro-ANP processing activity. Conditioned medium containing recombinant (Re) or endogenous (Endo) soluble (s) corin from HEK 293 (left) or HL-1 (right) cells were incubated with pro-ANP. Control medium and cell lysate with full-length (FL) corin were included as negative and positive controls, respectively. Pro-ANP to ANP conversion was analyzed by Western blotting. C, HEK 293 cells expressing corin or parental HEK 293 cells were incubated with increasing doses of GM6001. Soluble corin fragments (top) and pro-ANP processing activity (bottom) in the medium were analyzed by Western blotting. D, soluble corin fragments (top) and pro-ANP processing activity (bottom) in the conditioned medium from HEK 293 cells expressing WT corin and mutants R164A and R427A were analyzed by Western blotting. Data are from representative experiments that were repeated at least three times.