FIGURE 1.

The interaction between ATP7B and clusterin. Coimmunoprecipitation (co-IP) of ATP7B and clusterin from HepG2 cells (A), mouse liver tissues (B), HepG2 cells supplemented with CuCl₂ (0–200 μm for 24 h) (C), and HepG2 cells incubated with BCS/D-penicillamine (D-Pen; 0–500 μm for 72 h) (D). Proteins were fractionated and immunoblotted with anti-ATP7B, anti-(mouse) Atp7b, anti-clusterin, and anti-β-actin antibodies. Densitometric analysis of immunoprecipitated clusterin is shown (C(ii) and D(ii)), expressed as relative optical density and representing the mean ± S.E. (n = 3). Asterisks indicate values that are significantly different from control. *, p < 0.05.