FIGURE 3.

The interaction betwen ATP7A and clusterin. Coimmunoprecipitation (Co-IP) of ATP7A and clusterin from M17 cells supplemented with CuCl₂ (200 μm for 24 h) (A) (i) or BCS/D-penicillamine (D-Pen; 500 μm for 72 h) and H₂O₂ (0–2 mm for 20 min) or diamide (0–10 mm for 15 min) (B) (i). Cell lysates and coimmunoprecipitated proteins were fractionated by SDS-PAGE and immunoblotted with anti-ATP7A, anti-clusterin, and anti-β-actin antibodies. Densitometric analysis of immunoprecipitated clusterin is shown (A(ii) and B(ii)), expressed as relative optical density and representing the mean ± S.E. (n = 3). Asterisks indicate values that are significantly different from control. *, p < 0.05.