FIGURE 4.

Increased clusterin binding to unstable ATP7B mutants MBD1–6del and MBD3–5del. A, schematic representation of the N terminus of WT-ATP7B, MBD1–6del, and MBD3–5del. B (i), coimmunoprecipitation (Co-IP) of clusterin and WT-ATP7B, MBD1–6del, or MBD3–5del from CHO-K1 cells cultured at either 37 or 30 °C. Cell lysates and coimmunoprecipitated proteins were fractionated and immunoblotted with anti-ATP7B, anti-clusterin, and anti-β-actin antibodies. B (ii) densitometric analysis of immunoprecipitated clusterin relative to immunoprecipitated ATP7B is shown, expressed as relative optical density, and representing the mean ± S.E. (n = 3). Asterisks indicate values that are significantly different. *, p < 0.05.