FIGURE 4.

F3_Mhp107 binds fibronectin. Surface plasmon resonance was used to analyze binding of Mhp107 proteins to immobilized fibronectin. Mhp107 proteins were injected over the immobilized fibronectin with a flow rate of 10 μl/min for 600 s. The end of the injection period is indicated by an arrow, at which stage any dissociation of the Mhp107 proteins from the ligand can be observed. A, the sensorgrams of F3_Mhp107 at concentrations of 10 (bottom), 50, 100, 200, and 500 (top) nm binding fibronectin. B, sensorgrams of 200 nm F1_Mhp107 (top) and F2_Mhp107 (bottom) injected over immobilized fibronectin.