FIGURE 5.

F1_Mhp107 binds plasminogen. Surface plasmon resonance was used to analyze binding of Mhp107 proteins to immobilized porcine plasminogen. Mhp107 proteins were injected at a flow rate of 10 μl/min for 600 s. A, F1_Mhp107 was injected over the immobilized plasminogen at concentrations of 10 (bottom), 50, 100, 200, and 500 (top) nm. The end of the injection period is indicated by an arrow, at which stage dissociation of F1_Mhp107 from the ligand can be observed. B, sensorgrams of 200 nm F1_Mhp107 binding to immobilized plasminogen in the presence of 0, 10, 50, and 100 μm ϵ-aminocaproic acid. The analyte was injected with a flow rate of 10 μl/min for 600 s. C, sensorgrams of 200 nm F2_Mhp107 (top) and F3_Mhp107 (bottom) injected over immobilized plasminogen. The end of the injection period is indicated by an arrow.