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. 2011 Jan 21;286(12):10137–10146. doi: 10.1074/jbc.M110.205625

TABLE 2.

Oligonucleotides used in this work

Product Position Oligonucleotide (5′ to 3′)
MNE1 Forward TATTTAGGATCCATGAAGTTACTTTTTAAAAGATATTCGTCT
Reverse TATTTACTCGAGTTATTTTTGTTGCATTTGCTTAGATCTTAT
MNE1-Myc Forward TATTTAGGATCCATGAAGTTACTTTTTAAAAGATATTCGTCT
Reverse TATTTACTCGAGCTACAAGTCCTCTTCAGAAATGAGCTTTTGCTCGAGTTTTTGTTGCATTTGCTTAGA
mne1Δ::HIS3MX6 cassette Forward CGGCGAGAAAAAATGATAGTAGTGTGCCAAGAAGAATATGCGGATCCCCGGGTTAATTAA
Reverse CGGCAAATTTTACATATAATCATTATTTTTGTTGCATTTGCGAATTCGAGCTCGTTTAAAC
MNE1-13Myc::HIS3MX6 cassette Forward CTATTTTCTTACATAAGATCTAAGCAAATGCAACAAAAACGGATCCCCGGGTTAATTAA
Reverse GAGTCTGATATCACTTTATAGAATGTAAACGGCAAATTTTACGAATTCGAGCTCGTTTAAAC
COX2 exon fragment Forward GATTCGTTGTAACAGCTGCTGATG
Reverse GACCTGTCCCACACAACTCAG
COX1 E5β-E5γ Forward GCTCTAATCCATGGTGGTTCAATTAG
Reverse GAAAATGTCCCACCACGTAGTAAG
COX1 E5β-aI5β fragment Forward GCTCTAATCCATGGTGGTTCAATTAG
Reverse AATAATTATAAGAGTTTCCCCGTTAGC
COX1 aI5β-E5γ fragment Forward AAGAGATTATAAATCTGGTGCTACAGC
Reverse GAAAATGTCCCACCACGTAGTAAG
COX1 aI5β-pre-mRNA probe Forward AATTAACCCTCACTAAAGGGAAAGCTTAAGAGTAAAATTCTTAAAGTG
Reverse AATACGACTCACTATAGGGCGTCGACAAAATGTCCCACCACGTAGTAAGTC
COX1 E6 probe GAATAATGATAATAGTGCAATGAATGAACC
21S rRNA exon probe CAACATCAACCTGTTCGATCG
COX1 aI1 probe GTTTCTAATGTTGTACCTGGAG
COX1 aI2 probe GAATAAACAGAGATATGTTTATC
COX1 aI3 probe GCTAAATAAGGTCCCAACTTATC
COX1 aI4 probe CCATCACCATCAATTAATCCAGC
COX1 aI5α probe TCTATTTGATCTTGGATAAATATC
COX1 aI5β probe CCATCTCCTTCAAATAATCC
COX1 aI5γ probe GGTTTATTCTGTTTTATC