FIGURE 5.

MyD88-IRAK1/4 signaling pathway participated in the morphine treatment induced inhibitory IL-23 production. A and B, to inhibit MyD88-dependent signaling, cells were pretreated with MyD88 homodimerization inhibitory peptide or control peptide at 24 h before in vitro cell infection with S. pneumoniae (S. pn). C, BMDCs were preincubated with IRAK1/4 inhibitor (50 μm) or control vehicle 2 h before cell infection with S. pneumoniae. IL-23 concentration was measured in the supernatant by ELISA. **, p < 0.01, compared with the vehicle controls, or comparisons indicated by brackets. Results are representative of three independent experiments. ns, not significant.