TABLE 1.

Tag7 and HspBP1 secretion by LAK cells during interaction with K562

PBMC were incubated with IL2 (1000 units/ml) for 6 days. CD8+ lymphocytes were obtained with commercially available magnetic bead isolation kits and were incubated with K562 cells for 30 h (100:1) in serum-free RPMI 1640 at 37 °C. Cells were removed by centrifugation and the conditioned medium was tested for the presence of Tag7 and HspBP1. Concentrations of Tag7 and HspBP1 in the condition medium were determined with an immunoenzyme assay.

Time	Conc.Tag7	Conc.HspBP1	HspBP1/Tag7 ratio
h	nm	nm
1	0.055 ± 0.006	1.84 ± 0.2	33
3	0.095 ± 0.01	2.5 ± 0.2	26
6	0.15 ± 0.01	4.1 ± 0.3	27
18	0.34 ± 0.03	0.8 ± 0.1	2.3
30	0.4 ± 0.03	1.05 ± 0.1	2.6