FIGURE 3.

IL-1β and IGF-1 modulate CYP11B1 expression and cortisol production in HEK. A, IL-1 and ACTH stimulate cortisol production in HEK (p < 0.01), whereas IGF-1 inhibits it. Cells were incubated with IL-1β (10 ng/ml), IGF-1 (100 ng/ml), or ACTH (10⁻⁷ m) for 24 h (n = 5). Data represent the mean ± S.D. and were analyzed by one-way ANOVA (F = 75.32; p < 0.0001) and Dunnett's post hoc test (*, p < 0.01). B, IL-1 stimulates CYP11B1 expression in HEK, and IGF-1 inhibits it. Cells were incubated with IL-1β (10 ng/ml), IGF-1(100 ng/ml), or ACTH (10⁻⁷ m) for 24 h (n = 3). Quantitative real time PCR for the expression of CYP11B1 is shown. Expression levels were normalized to HPRT1. Error bars represent S.D.