FIGURE 5.

Expression of CYP11B1 and cortisol synthesis during acute wound healing in vivo. A, CYP11B1 gene expression determined using real-time qPCR showed induction at 48 h post wounding as compared with normal porcine skin (n = 3). Error bars represent S.D. B, laser microdissected epidermal keratinocytes demonstrate induction of CYP11B1 enzyme 48 h post wounding. At the later time points CYP11B1 expression decreased back to the level of normal, unwounded skin (n = 3). Error bars represent S.D. C, porcine wound explants show the highest levels of cortisol production 48 h after the wounding (n = 2). Cortisol production is inhibited with metyrapone (10⁻³ m) and induced by ACTH (10⁻⁷ m).