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. 2011 Jan 19;286(12):10297–10304. doi: 10.1074/jbc.M110.203729

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — SCCRO rescues nuclear localization and neddylation of Cul1-Δ610–615. A, immunoblot on lysates from U2OS cells transfected with the indicated constructs and probed with anti-Myc or anti-HA antibodies following HA immunoprecipitation showing increased interaction between Roc1 and HA-Cul1-Δ610–615 with SCCRO co-expression (compare lanes 4 with 2 and 6). B, immunoblot on lysates from U2OS cells transfected with the indicated constructs probed with anti-HA antibodies showing rescue of HA-Cul1-Δ610–615 neddylation defect by SCCRO (top panel, lanes 2 and 6). C, immunofluorescence on U2OS cells co-transfected with FLAG-Cul1-Δ610–615 and HA-NES-SCCRO using Cy3-conjugated anti-FLAG antibody and FITC-conjugated HA antibody showing nuclear exclusion of SCCRO and loss of nuclear translocation of Cul1-Δ610–615.