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Strategy for genetic modifications. A–D, construction of targeted gene replacement strains and placement of primers for verification of the strains: A, ΔaurZ; B, ΔaurS; C, ΔaurT; D, ΔaurZ/J. The figure is not drawn to scale. Primer sequences are listed in supplemental Table S1. The size of the deletion is listed as the number of removed base pair positions when compared with the size of the coding sequence.
