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Binding of NLS-containing proteins to Hu and Av Imp-α1. A, Imp-α1 (1–100 mm) was injected over immobilized proteins containing the bipartite NLS peptide and additional NLS domain, 627-NLS domain or MBP. B–D, histograms showing values of KD, kon, and koff, respectively. Error bars indicate the average of two separate experiments.
