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. 2011 Jan 28;286(12):10449–10456. doi: 10.1074/jbc.M110.209510

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — MafA interacts with ATF2. A, HeLa cells were transfected with expression plasmids for FLAG-tagged wild-type (WT) ATF2 or an L23P mutant of ATF2 (schematically indicated at the top) with or without an expression plasmid for HA-MafA. Cell extracts were subjected to immunoprecipitation with an anti-HA antibody, and the immune complexes (α-HA I.P.) were analyzed by immunoblot using an anti-HA or anti-FLAG antibody. As a control, total cell extract (input) was also analyzed. B, reciprocal immunoprecipitation experiment using EGFP-tagged MafA or an L23P variant of MafA (indicated schematically at the top) and FLAG-ATF2. C, ATF2, ATF7, and CRE-BPa were synthesized in vitro in the presence of [³⁵S]methionine and used in a GST-pull down assay with glutathione-Sepharose-immobilized GST or GST-MafA (indicated schematically at the top). Precipitates were analyzed by SDS-PAGE followed by autoradiography.