FIGURE 3.

Lys¹¹, Lys⁴⁸, and Lys⁶³, but not Lys²⁹ linkages accumulate in frontal cortex of AD or AD/LBD specimens. A–H, pathological staining of α-synuclein (Lewy body), amyloid β peptide (plaques), Tau (neurofibrillary tangles), and Lys⁶³-linked polyUb in control and AD/LBD cases, respectively. Wide-field images were captured with a 10× objective, except a 40× objective was used for D and H. Scale bar is 150 μm for all panels but D and H (20 μm). I, elevated level of Ub in AD/LBD cases in Western blotting. J, analysis of four abundant polyUb linkages in total frontal cortex lysate of the matched control (n = 12), pure AD (n = 12), and AD/LBD cases (n = 12) by LC-MS. The asterisk indicates p value <0.05 according to Student's t test.