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. 2011 Jan 13;286(12):10466–10475. doi: 10.1074/jbc.M110.214726

FIGURE 5.

FIGURE 5.

CrV2-Alexa interacts only minimally with Gβγ-Tb. 10 nm Gβγ-Tb was mixed with 20 nm CrV2-Alexa or 10 nmi1-Alexa to a final volume of 100 μl with TMN buffer. TR-FRET measurements were taken with the following parameters: λex 340 nm, λem 572 nm, 50-μs delay, and 900-μs counting duration over the time period shown. Data shown are mean (n = 2).