FIGURE 6.
Alteration of the activation state of Gαi1 using aluminum fluoride decreases the interaction with CrV2. 10 nm Gαi1-Tb was mixed with 20 nm CrV2-Alexa with excess amounts of GDP (2.5 μm) or aluminum fluoride produced by the addition of NaF (10 mm) and AlCl3 (30 μm). TR-FRET measurements were immediately taken with the following parameters: λex 340 nm, λem 572 nm, 50-μs delay, and 900-μs counting duration. Background of Gαi1-Tb with GDP or aluminum fluoride has been deducted. Data shown are mean ± S.E. (n = 3). a.u., arbitrary units.