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. 2010 Dec 29;286(12):10515–10529. doi: 10.1074/jbc.M110.176271

FIGURE 4.

FIGURE 4.

Side views of wild type and Trp(E7) mutants of HbA and Mb. The electron density was contoured at 1.3 σ and is shown as a blue net. The color code for atoms is as follows: green, carbon; blue, nitrogen; red, oxygen; orange, iron. The key residues are shown clockwise: Trp(E7) or His(E7), His(F8), Val(E11), and Leu(B10). The hemoglobin subunits are part of a tetrameric structure with wild type or native subunit partners. A, top panels, native deoxy-α (55) (Protein Data Bank code 2DN2) with the distal pocket water removed and Trp-58(E7) deoxy-α (Protein Data Bank code 3NMM); bottom panels, native CO-β (55) (Protein Data Bank code 2DN3) and Trp-63(E7) βCO (Protein Data Bank code 3NL7). B, top panels, wild type deoxyMb (49) (Protein Data Bank code 2MGL) with the distal pocket water removed and Trp-64(E7) deoxyMb (Protein Data Bank code 3OGB); bottom panels, wild type MbCO (49) (Protein Data Bank code 2MGK) and Trp-64(E7) MbCO (Protein Data Bank code 3NML).