TABLE 2.

Kinetic parameters for human RNase H2 with and without AGS mutations

	Km	kcat	kcat/Km
	nm	min−1	min−1nm−1
D14R1D3:DNA18
Wild type	28 ± 2.7a	24 ± 0.7	0.9
A:R291H	27 ± 1.8	9.5 ± 0.2	0.3
C:K143I	30 ± 3.4	21 ± 0.8	0.7
RNA18:DNA18
Wild type	143 ± 1.7	209 ± 0.8	1.5
A:R291H	144 ± 4.5	59 ± 0.6	0.4
C:K143I	158 ± 3.4	126 ± 0.9	0.8

^a Values indicate standard errors.