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Thermal stability of human RNase H2 with selected AGS mutations (Thermofluor assay)
| Protein | Tm | ΔTm (compared with wild type) |
|---|---|---|
| °C | °C | |
| Wild type | 51.1 ± 0.1 | |
| B:W73L | 45.9 ± 0.3 | −5.2 |
| B:G83S | 45.7 ± 0.2 | −5.4 |
| B:H86R | 44.2 ± 0.1 | −6.9 |
| B:Y219H | 44.8 ± 0.1 | −6.3 |
