FIGURE 1.

Sequence, expression, and location of ARL16. A, alignment of human, mouse, Xenopus, and Drosophila ARL16 amino acid sequences. B, alignment of human ARL16 with ARF1 and ARL1 amino acid sequences. C, mouse anti-ARL16 serum specifically recognizes Flag-ARL16 and endogenous ARL16, but not Flag-tagged ARF1 and ARL1. D, expression of ARL16 in mammalian cells. Lysates of the indicated cells were analyzed by Western blot with anti-ARL16 serum. E, ARL16 localization in HEK293 and HeLa cells. Upper panels: HEK293 cells transfected with an expression plasmid for Flag-tagged ARL16; immunofluorescent staining was performed with anti-Flag (αFlag) or mouse IgG; untransfected HEK293 cells stained with preimmune serum (preserum) or anti-ARL16 serum (αARL16); lower panels: transfected and untransfected HeLa cells stained as in the upper panels. The experiments were repeated three times, and similar results were obtained.