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. 2011 Jan 3;286(12):10581–10592. doi: 10.1074/jbc.M110.187450

FIGURE 1.

FIGURE 1.

Cell-surface binding of monovalent TatP. A, spatial precision of TatP-QDs in HeLa. Immobile TatP-QDs were tracked in HeLa. The S.D. of the position of QDs was 6.9 nm in the x axis and 6.5 nm in the y axis. B, typical time course of monovalent TatP-QD cell binding to HeLa cells pre-exposed to 30 pm of monovalent Tat-QDs, followed by exposure to the indicated concentrations of nonlabeled TatP or medium. Data were collected at 400 ms/frame. Arrow indicates time of TatP addition. C, HeLa were exposed to either FITC-TatP, FITC alone (control) (upper panel), 1- and 4-val TatP-Alexa488, or Alexa488 alone (lower panel) for 15 min and analyzed for their binding capability by FACS. D, schematic figure illustrates the experiments for E–G: monovalent TatP-QD contains free sites for biotin-TatP on the Sts. E and D, typical time course of TatP-QD cell binding (E) and selected frames (F) from HeLa cells pre-exposed to 30 pm monovalent Tat-QDs, followed by exposure to the indicated concentrations of biotin-TatP. Arrows in E and F (right panel) indicate the time of biotin-TatP addition. Bars, 5 μm. F, arrowheads (left panel) indicate filopodia. G, mean QD number/cell calculated from ∼20 cells exposed to biotin-TatP for 15 min. Bars, S.D. Results are representative of three independent experiments.