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. 2011 Jan 18;286(12):10605–10617. doi: 10.1074/jbc.M110.211466

FIGURE 7.

FIGURE 7.

Mitochondrial PGC-1α is in a complex with Tfam, an association that is positively modulated by acute exercise. A, PGC-1α was co-immunoprecipitated (IP) followed by immunoblotting (IB) for Tfam to assess the PGC-1α·Tfam complex content in mitochondrial fractions prepared from skeletal muscle (quadriceps femoris) of END and END+3h compared with SED mice (n = 8/group). B, Tfam was co-immunoprecipitated followed by immunoblotting for PGC-1α to assess PGC-1α·Tfam complex content in mitochondrial fractions prepared from skeletal muscle (quadriceps femoris) of END and END+3h compared with SED mice (n = 8/group). Anti-IgG antibody was used as a nonspecific control for mitochondrial co-immunoprecipitation assay. **, p < 0.01, versus SED; †, p < 0.05 versus both END and SED. Error bars represent S.E. C, cytosolic PGC-1α does not form a complex with Tfam. PGC-1α was co-immunoprecipitated followed by IB for Tfam to assess PGC-1α·Tfam complex content in the S100 cytosolic fractions prepared from skeletal muscle (quadriceps femoris) of END and END+3h compared with SED mice (n = 8/group). Anti-IgG antibody was used as a nonspecific control for cytosolic co-immunoprecipitation assay. S100 cytosol fraction (CYT) was utilized as a positive control for Tfam protein via immunoblotting only. LDHA, lactate dehydrogenase.