TABLE 1.

Agonist activities of small carboxylic acids on hFFA2 and hFFA3, calculated ligand efficiencies, and average selectivities

Data are mean ± S.E., n ≤ 3.

^a DMR assay using the Corning Epic biosensor.

^b LE is calculated by the formula −Δg = −ΔG/N_{non-hydrogen atoms} where −ΔG = RTln(K_D) presuming that K_D ≈ EC₅₀. The average of pEC₅₀ values from at least two different assays was used in the calculation. Values are given as kcal mol⁻¹ atom⁻¹.

^c Selectivity (calculated by ΔpEC₅₀ = pEC_50,FFA2 − pEC_50,FFA3) is based on the average pEC₅₀ values of the available assays.

^d n.a., not assessed.