TABLE 2.
DNA binding affinities for WT Zn1 and Zn2 domains and mutants
| Zn1 domain | KD | KDa | Zn2 domain | KD |
|---|---|---|---|---|
| μm | μm | μm | ||
| WT | 1.96 ± 0.54 | 0.19 ± 0.03 | WT | 0.032 ± 0.004 |
| Phosphate backbone grip mutants | ||||
| R18A | >30 | >30 | R122A | 2.58 ± 1.15 |
| R34A | >30 | >30 | R138A | >8 |
| Base stacking loop mutants | ||||
| F44A | >30 | 1.04 ± 0.33 | L151A | 0.115 ± 0.035 |
| V48A | 10.2 ± 1.3 | 2.05 ± 1.16 | I154A | 0.829 ± 0.117 |
| F44A/V48A | >30 | 5.16 ± 0.72 | L151A/I154A | >8 |
| Q40A | 3.93 ± 0.86 | 0.24 ± 0.03 | ||
| D45A | 2.08 ± 0.54 | 0.10 ± 0.01 | ||
a Experiment performed in lower ionic strength buffer. See text and “Experimental Procedures” for details.