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Detection of a PilQ complex in T. thermophilus HB27. A and B, 3–12% polyacrylamide gradient SDS-PAGE of crude extracts of HB27 wild type (wt) and pilQ mutant (pilQ::kat) (A) followed by immunoblotting with antiserum against PilQ (B). C, membranes of HB27 were treated with 10% SDS and incubated at 100 °C. Samples were taken at the indicated time points and subjected to Western blot analyses with PilQ antibodies.
