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Subunit composition of the PilQ complex. A and B, the purified PilQ complex was treated with hot phenol and subjected to 3–12% polyacrylamide gradient SDS-PAGE (A) and immunoblotting (B). Lane 1, untreated PilQ complex; lane 2, PilQ complex treated with hot phenol. C, the purified untreated PilQ complex was subjected to Blue native-PAGE followed by immunoblotting.
