FIGURE 6.
DNA binding of the PilQ complex measured by bandshift analyses. Reaction mixtures contained 1 nm (30,000 cpm) of dsDNA. DNA was incubated with 5, 20, 50, 100, and 200 nm purified PilQ complex at 37 °C (A) and 68 °C (B), respectively. Competitor DNA was added to 200 nm PilQ complex in 20-fold excess (lane C). 200 nm BSA was used as negative control.