Secondary structure prediction of secretins. The PredictProtein server was used to predict potentially β-sheet (gray boxes) and α-helical (black boxes) regions within the secretins shown. Conserved C-terminal domains are indicated as dark gray boxes A, B, C, and D (61) for PilQ of T. thermophilus; the processing site is indicated by an arrow. T. t., T. thermophilus; K. o., K. oxytoca; E. c., E. coli; P. a., Pseudomonas aeruginosa; S. e., Salmonella enterica; Y. p., Yersinia pestis; EPEC, enteropathogenic E. coli; BFP, bundle-forming pilus system; N. m. Neisseria meningitidis.