Figure 4.
(A and B) Semi-quantitative RT-PCR analyses of the genes cyp26A1, cyp26B1, and trim31 for validation of microarray. 24-hour treatment samples. A representative experiment for each gene is shown. A housekeeping gene, gapdh, was used as an internal control. A sample without reverse transcription, (NO RT) was used as a negative control in the PCR reactions (not shown). These experiments were repeated three times with different RNA preparations and similar results were obtained. Data are shown for (A) cyp26a1 and cyp26b1; and (B) for trim31.
