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. Author manuscript; available in PMC: 2011 Mar 18.
Published in final edited form as: Cancer Biol Ther. 2008 Oct 9;7(10):1607–1618. doi: 10.4161/cbt.7.10.6584

Figure 5.

Figure 5

Semi-quantitative RT-PCR analyses of selected genes cyp26A1, cyp26B1, il1β, gata3, phlda1, ceacam5, txk and gapdh for validation of microarray results: 24-hour treatment samples. A representative experiment for each gene is shown. Gapdh was used as an internal control. A sample without reverse transcription, (NO RT), was used as a negative control in the PCR reactions. These experiments were repeated three times with different RNA preparations and similar results were obtained. (A) PCR results for il1ß, gata3, phlda1, ceacam5, txk, and gapdh are shown. (B) Gata3 mRNA levels in SKRC06 and SKRC39 relative to HK-2 cells; (C) vhl3 mRNA levels in HK-2, SKRC06 and SKRC39 cells.