Figure 1. Generation of TRPM7-deficient mice.

(a) A schematic representation of TRPM7 protein. Arrow indicates the position of truncation in TRPM7^Δkinase mice. Also shown are the structures of the murine TRPM7 locus, targeting vector and resulting homologous recombination locus. (b) Southern blot analysis of genomic DNA from wild-type (+/+) or TRPM7^+/Δkinase (+/−) mice after digestion with NcoI. (c) TRPM7^+/Δkinase (+/−) and TRPM7^{Δkinase/Δkinase} (−/−) embryos at 7.5 days post coitum. Staining with Brachyury antisense mRNA marks the primitive streak, where mesoderm formation occurs, and demonstrates that embryos are at the advanced primitive streak stage. Scale bar, 100 μm.