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. 2010 Nov 2;1:109. doi: 10.1038/ncomms1108

Figure 4. TRPM7 (MagNuM) currents in ES cells derived from TRPM7 deficient mice.

Figure 4

(a) Average normalized time course of TRPM7 current development in mouse ES cells isolated from wild-type (black circles, n=13), TRPM7+/Δkinase (red circles, n=12) and TRPM7Δkinase/Δkinase mice (blue circles, n=13). Data were acquired and analysed as in Figure 3a. Error bars indicate s.e.m. The intracellular solution was divalent-free and contained (in mM) 140 Cs-glutamate, 8 NaCl, 10 Cs-EGTA, 5 Na-EDTA (pH 7.2, 300 mOsm). The extracellular solution contained (in mM) 140 NaCl, 1 CaCl2, 2.8 KCl, 2 MgCl2, 10 HEPES–NaOH, 11 glucose (pH 7.2, 300 mOsm). The application solution contained (in mM) 150 mM NaCl, 2.8 KCl, 10 HEPES–NaOH, 0.5 Na-EDTA. (b) Average current–voltage (I/V) curves from a representative wild-type cell (black), TRPM7+/Δkinase (red) and TRPM7Δkinase/Δkinase (blue) extracted at 200 s. (c) Average normalized time course of MagNuM current development in mouse ES cells isolated from wild-type (black circles, n=8), TRPM7+/Δkinase mice (red circles, n=10) and TRPM7Δkinase/Δkinase mice (blue circles, n=10). Data were acquired and analysed as in a. At the time indicated by the black bar, cells were superfused with external solution supplemented with 50 μM 2-APB. (d) Current–voltage (I/V) curves extracted from a representative wild-type cell (black), TRPM7+/Δkinase (red) and TRPM7Δkinase/Δkinase (blue).