Fig. 4.

ER, HSP 25, 32, and 90 expression in adult and old myocytes. A, Graph summarizes the basal ER-α and β expression in cardiac myocytes from adult and old rats with or without in vivo E₂ replacement. ER expression was normalized to GAPDH expression and to adult ovx. Right panel, Representative western blots. n = 3 animals and 3–5 plates per group. Ctl, control. B, Representative Western blots of HSP 25, 32, 90, and GAPDH expression in adult and old rats treated with 5 and 100 nm E₂ for 12 h in vitro. n = 6 animals and 6–11 plates per group. P = n.s., data not shown.