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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Integr Plant Biol. 2010 Dec 22;53(1):54–62. doi: 10.1111/j.1744-7909.2010.01007.x

Figure 1. Cotyledon vascular patterns of yuc4 enhancers.

Figure 1

(A) Wild type seedlings displayed four-loop vein pattern.

(B and C) Seedlings of yuc1 yuc4 double mutants (T-DNA insertion lines). Note that the yuc1 yuc4 double mutants made fewer veins. Some of the veins were not continuous. Vascular patterns were variable in the yuc1 yuc4 double mutants, but they never formed lobes observed in wt cotyledons.

(D to H) Seedlings of yuc4 enhancers. The enhancers were new yuc1 alleles (see text for details). Seedlings of yuc1–4 yuc4 (D), yuc1–5 yuc4 (E), yuc1–6 yuc4 (F), yuc1–7 yuc4 (G), and yuc1–8 yuc4 (H) are shown. Seedlings were grown for 7 days under long day conditions. Seedlings were cleared using acetic acid/ethanol treatments followed by chloral hydrate treatments.