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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Mol Cell. 2011 Feb 18;41(4):409–418. doi: 10.1016/j.molcel.2011.01.022

Figure 2. GAL4-p53 stimulates transcription-coupled polyadenylation.

Figure 2

(A) Purification of bacterially expressed GAL4-p53. His-tagged GAL4-p53 was purified by using talon resin and 5 μg resolved by SDS-PAGE.

(B) Transcription-polyadenylation assay with or without GAL4-p53. Transcripts produced in NE were analyzed on 5% denaturing gel and quantitated as in Fig. 1C.