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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Mol Cell. 2011 Feb 18;41(4):409–418. doi: 10.1016/j.molcel.2011.01.022

Figure 6. GAL4-VP16 recruits PAF1c to DNA templates.

Figure 6

(A) Immobilized template assay to analyze protein recruitment. Biotinylated DNA templates were immobilized using streptoavidin magnetic beads (Invitrogen) and incubated with NE alone or supplemented with GAL4-VP16 in the presence or absence of NTPs as indicated under transcription-coupled polyadenylation conditions. After extensive washing, proteins bound to the templates were eluted and analyzed by western blotting with the indicated antibodies. Protein bands were detected and quantitated with LI-COR Odyssey. Error bars represent standard deviations from three independent experiments.

(B) Immobilized template assay with mutant templates lacking GAL4 binding sites. Assay were done and analyzed as in (A).