Table 1.

Basal values of RWC%, ROS (i.e. H₂O₂ and superoxide anions), lipoperoxide, and carbonyl content determined in naturally hydrated, naturally dehydrated, in vitro dehydrated, and in vitro rehydrated P. columbina plants

	Hydrated plants	Dehydrated plants in vivo/in vitro	Rehydrated plants
RWC (%)	100	3.55±2.8*/5.41±4.8*	94.8±2.8ns
ROS
Hydrogen peroxide (nmol DCF g−1 FT)	0.21±0.1	2.66±0.9*/2.44±0.6*	0.47±0.1 ns
Superoxide anions (nmol 2OH-E g−1 FT)	0.79±0.5	3.75±0.6*/4.05±0.6*	1.52±0.3 ns
Lipid oxidation
Lipoperoxide (nmol g−1 DT)	70.20±11.5	74.8±24.9 ns/72.4±13.3 ns	68.3±5.8 ns
Protein oxidation
Carbonyl content (nmol mg−1 protein)	0.26±0.1	1.16±0.3*/1.00±0.3*	0.21±0.1 ns

Each value is an average of three independent replicates±1 SD.

DCF, 2,4 dichlorofluoresceine; ^ns not significant.

*Significant (P<0.05) differences with values measured in hydrated plants.