Table 3.
Summary of genome-wide linkage disequilibrium analysis
Market class | No. marker pairsa |
r2 estimatesb |
Linkage disequilibrium decay (cM)e |
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Median | St. Dev. | 95th percentilec | P <0.01d | LOESSf |
Moving meansg |
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95th percentile method | Fixed r2 (0.1) method | 95th percentile method | Fixed r2 (0.1) method | ||||||
Combinedh | 5248 | 0.011 | 0.102 | 0.160 | 8.1% | 6.6 | 8.0 | 6 | 6 |
Processing | 3294 | 0.037 | 0.131 | 0.248 | 5.5% | 6.9 | 14.2 | 6 | 9 |
Fresh market | 2622 | 0.031 | 0.187 | 0.464 | 2.0% | 3.0 | 16.1 | 2 | 10 |
The number of marker pairs includes only markers polymorphic within each market class.
Linkage disequilibrium was estimated as r2 values for all possible marker pairs using TASSEL (Bradbury et al., 2007) and GGT (van Berloo, 2008) software.
The 95th percentile of the distribution of r2 values for the unlinked markers. This value is the baseline r2 to estimate LD decay.
Percentage of r2 estimates with P value <0.01. P values of r2 estimates were calculated from 1000 permutations using TASSEL software (Bradbury et al., 2007).
Linkage disequilibrium decay was estimated over genetic distance by the relationship of a baseline r2 estimate to linked marker pairs using two methods, LOESS and 1 cM moving means. The baseline r2 value was either fixed at 0.1 or estimated using the 95th percentile of the unlinked markers. Values for r2 that exceed the baseline are considered to be in linkage disequilibrium.
For the LOESS estimation of LD decay, genetic distance was estimated as the point where the LOESS curve first crosses the baseline r2 value.
For the means estimation of LD decay, the r2 values of linked markers were grouped into bins of 1 cM based on the distance between markers. LD decay was estimated as the first bin where the baseline r2 value was lower than the bin mean.
The combined analysis includes processing, fresh market, and vintage cultivars.