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Semi-quantitative PCR for VRNH1 (30 cycles), VRNH2 (35 cycles), VRNH3 (35 cycles), PPDH1 (30 cycles), PPDH2 (35 cycles), and Actin (30 cycles) in three lines of barley vernalized under conditions of a short photoperiod (VSD), in the course of 6 weeks (0, 7, 14, 21, 28, 35, and 42 d).
