Table 3.
DNA methylation changes in leaf of DK151 and IR64 at booting and heading stages
Growth stage | Booting |
Heading |
||||||||||
Genotype | DK151 |
IR64 |
DK151 |
IR64 |
||||||||
MSAP band type | Control | Stress | Recovery | Control | Stress | Recovery | Control | Stress | Recovery | Control | Stress | Recovery |
I | 888 | 886 | 889 | 899 | 901 | 899 | 891 | 901 | 888 | 898 | 899 | 898 |
II | 79 | 83 | 82 | 78 | 79 | 80 | 81 | 84 | 82 | 78 | 82 | 76 |
III | 166 | 167 | 164 | 166 | 164 | 166 | 166 | 164 | 166 | 166 | 165 | 166 |
IV | 44 | 41 | 42 | 13 | 12 | 11 | 39 | 28 | 41 | 14 | 10 | 16 |
Total amplified bands | 1177 | 1177 | 1177 | 1156 | 1156 | 1156 | 1177 | 1177 | 1177 | 1156 | 1156 | 1156 |
Total methylated bandsa | 289 | 291 | 288 | 257 | 255 | 257 | 286 | 276 | 289 | 258 | 257 | 258 |
MSAP (%) | 24.55 | 24.72 | 24.47 | 22.23 | 22.06 | 22.23 | 24.3 | 23.45 | 24.55 | 22.32 | 22.23 | 22.32 |
Fully methylated bandsb | 210 | 208 | 206 | 179 | 176 | 177 | 205 | 192 | 207 | 180 | 175 | 182 |
Full methylated ratio (%) | 17.84 | 17.67 | 17.5 | 15.48 | 15.22 | 15.31 | 17.42 | 16.31 | 17.59 | 15.57 | 15.14 | 15.74 |
Type II are hemi-methylated bands and types III+IV are fully methylated bands. Total methylated bands = II+III+IV.