Fig. 1.

Relative expression of PsCab, PsTRX f, and PsTRX m1 genes in leaves of pea during the day/night cycle. Plants were grown under a 12 h light/12 h night photoperiod, and leaf samples were collected at 3 h intervals over a period of 45 h. Total RNA was extracted and analysed by northern blotting (15 μg). Filters were hybridized with 3'-specific probes of PsCab, PsTRX f and PsTRX m1 genes, and loading differences were corrected by re-probing the filter with a cDNA fragment of the 18S rRNA. Clear areas in the graph indicate the day during the day/night periods, the grey area indicates the night, and the hatched area indicates the night period in which the light was kept on. The graph shows quantification of northern blot data normalized considering the lowest value in each case. Numbers on the ordinates indicate the real time in hours at which samples were collected for RNA isolation. (Fisher's exact test, P=0.01). (This figure is available in colour at JXB online.)