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. 2011 Jan 5;62(6):2053–2062. doi: 10.1093/jxb/erq395

Fig. 3.

Fig. 3.

Cloning of BC6. (A) Map-based cloning using an F2 population of ∼1000 plants. The BC6 locus was narrowed to a 170 kb region of chromosome 9, flanked by markers 5579-54kb and 5420-101kb. BAC clones AP005579 and AP005420 encompass the OsCesA9 gene. The Bc6 mutant had the missense mutation, R588G, in the OsCesA9 gene. Black and white boxes indicate coding and untranslated regions, respectively. (B) Alignment of amino acid sequences of OsCesAs and OsCsls. The sequence of the highly conserved region of the second cytoplasmic domain of OsCesA9 was aligned with the corresponding regions of other OsCesAs and OsCsls by using the ClustalW program. Residues conserved among all OsCesA members are shown in black. The asterisk indicates the residue mutated in Bc6. An arrowhead indicates the residue corresponding to P557 of AtCesA7, which is substituted with threonine by semi-dominant fra5 mutation. Accession numbers for the sequences of OsCesAs and OsCsls are as follows: OsCesA1, Os05g0176100; OsCesA2, Os03g0808100; OsCesA3, Os07g0424400; OsCesA5, Os03g0837100; OsCesA6, Os07g0252400; OsCesA8, Os07g0208500; OsCslA1, Os02g0192500; OsCslC1, Os01g0766900; OsCslD1, Os10g0578200; OsCslE1, Os09g0478100; OsCslF1, Os07g0553000; OsCslH1, Os10g0341700.