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. 2010 Dec 31;62(6):2155–2168. doi: 10.1093/jxb/erq413

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5 — Roscovitine reduces and colchicine increases the amounts of MsRBR and phospho-MsRBR proteins in alfalfa cells exposed to double synchronization. (A) Frequency data of G₁, S, and G₂ cells at various time points after removal of the inhibitor [hydroxyurea (HU) at 10 mM] and treatment with roscovitine or colchicine for the indicated time periods. Characteristic DNA histograms from flow cytometry are also shown. (B) Western blot of protein extracts with anti-AtRBR1 antibody (upper) and anti-human pRb phosphopeptide antibody (low). C, control, non-synchronized culture; BW, before washing out HU; AW, after washing out HU.