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. 2001 Mar 13;98(6):3062–3067. doi: 10.1073/pnas.051620698

Figure 2.

Figure 2

(A) Melting behavior of a fluorescent G-rich oligonucleotide (F21T, 0.2 μM strand concentration) alone (crosses) or in the presence of compound 1 (●, 0.5 μM; ■, 1 μM; ⧫, 2 μM,; ▴, 3 μM). Excitation was set at 470 nm, and fluorescence emission (arbitrary units) was recorded at 515 nm. Buffer was 0.1 M LiCl, 10 mM sodium cacodylate, pH 7.3. (B) Melting behavior of a G-rich oligonucleotide (F21T, 0.2 μM strand concentration) in the presence of a 100× molar excess of a double-stranded oligonucleotide (crosses) but in the absence of any ligand (the curve is nearly identical to that observed in the absence of double-stranded DNA, see A). The three other curves were obtained in the presence of compound 1 (1 μM) with no competitor (■) or a 43× excess of dS26, a double-stranded competitor (●) or a 100× excess (⧫). Excitation was set at 470 nm, and fluorescence emission was recorded at 515 nm. Buffer was 0.1 M LiCl, 10 mM sodium cacodylate, pH 7.3.